Generation of human embryonic stem cell line chHES-458 from abnormal embryos with HTT gene mutation

Pingyuan Xie; Yanfang Sun; Xiaoying Zhou; Jing Chen; Juan Du; Yi Sun; Guangxiu Lu; Ge Lin; Qi Ouyang

doi:10.1016/j.scr.2016.11.002

Generation of human embryonic stem cell line chHES-458 from abnormal embryos with HTT gene mutation

Stem Cell Res. 2016 Nov;17(3):627-629. doi: 10.1016/j.scr.2016.11.002. Epub 2016 Nov 5.

Authors

Pingyuan Xie¹, Yanfang Sun², Xiaoying Zhou³, Jing Chen⁴, Juan Du⁵, Yi Sun⁶, Guangxiu Lu⁷, Ge Lin⁸, Qi Ouyang⁹

Affiliations

¹ Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; National Engineering and Research Center of Human Stem Cell, Changsha, China.
² Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China.
³ National Engineering and Research Center of Human Stem Cell, Changsha, China.
⁴ Reproductive & Genetic Hospital of CITIC - Xiangya, Changsha, China.
⁵ Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, China; Reproductive & Genetic Hospital of CITIC - Xiangya, Changsha, China.
⁶ Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; National Engineering and Research Center of Human Stem Cell, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, China.
⁷ National Engineering and Research Center of Human Stem Cell, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, China; Reproductive & Genetic Hospital of CITIC - Xiangya, Changsha, China.
⁸ Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; National Engineering and Research Center of Human Stem Cell, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, China; Reproductive & Genetic Hospital of CITIC - Xiangya, Changsha, China.
⁹ Institute of Reproductive and Stem Cell Engineering, School of Basic Medical Science, Central South University, Changsha, China; National Engineering and Research Center of Human Stem Cell, Changsha, China; Key Laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha, China. Electronic address: loretta0730@163.com.

PMID: 27934595
DOI: 10.1016/j.scr.2016.11.002

Abstract

The human embryonic stem cell (hESC) line chHES-458 was derived from a abnormal blastocyst carrying the expanded CAG repeat mutation of the HTT gene that would lead to Huntington disease. This cell line maintained a normal karyotype 46, XX during long-term culture and displayed pluripotent characteristics, including expression of pluripotency-related transcription factors and capacity of forming well-differentiated three germ layers after being injected into the SCID mice.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Blastocyst / cytology
Cell Differentiation
Cell Line
Cellular Reprogramming
Embryoid Bodies / cytology
Embryoid Bodies / metabolism
Female
Human Embryonic Stem Cells / cytology*
Human Embryonic Stem Cells / metabolism
Human Embryonic Stem Cells / transplantation
Humans
Huntingtin Protein / genetics*
Huntington Disease / genetics
Huntington Disease / pathology*
Karyotype
Mice
Mice, SCID
Microscopy, Fluorescence
Plasmids / genetics
Plasmids / metabolism
Teratoma / pathology
Transcription Factors / genetics
Transcription Factors / metabolism
Trinucleotide Repeats / genetics

Substances

HTT protein, human
Huntingtin Protein
Transcription Factors