Gene Expression Analysis of Cultured Rat-Endothelial Cells after Nd:YAG Laser Irradiation by Affymetrix GeneChip Array

In Vivo. 2017 Jan 2;31(1):51-54. doi: 10.21873/invivo.11024.

Abstract

Endothelial cells and dental pulp cells enhance osteo-/odontogenic and angiogenic differentiation. In our previous study, rat pulp cells migrated to Nd:YAG laser-irradiated endothelial cells in an insert cell culture system. The purpose of this study was to examine the possible changes in the gene expression of cultured rat aortic endothelial cells after Nd:YAG laser irradiation using affymetrix GeneChip Array. Total RNA was extracted from the cells at 5 h after laser irradiation. Gene expressions were evaluated by DNA array chip. Up-regulated genes were related to cell migration and cell structure (membrane stretch, actin regulation and junctional complexes), neurotransmission and inflammation. Heat-shock 70 kDa protein (Hsp70) was related to the development of tooth germ. This study offers candidate genes for understanding the relationship between the laser-stimulated endothelial cells and dental pulp cells.

Keywords: Affymetrix GeneChip Array; Nd:YAG laser; endothelial cell.

MeSH terms

  • Animals
  • Aorta / cytology
  • Aorta / metabolism*
  • Aorta / radiation effects
  • Biomarkers / metabolism*
  • Cells, Cultured
  • Endothelium, Vascular / cytology
  • Endothelium, Vascular / metabolism*
  • Endothelium, Vascular / radiation effects
  • Gene Expression Profiling*
  • Gene Expression Regulation / radiation effects*
  • Lasers*
  • Oligonucleotide Array Sequence Analysis / methods*
  • Rats

Substances

  • Biomarkers