Mono-functionalized glycosylated platinum(IV) complexes possessed both pH and redox dual-responsive properties: Exhibited enhanced safety and preferentially accumulated in cancer cells in vitro and in vivo

Eur J Med Chem. 2017 Mar 10:128:45-55. doi: 10.1016/j.ejmech.2017.01.032. Epub 2017 Jan 23.

Abstract

A serious of carbohydrate-conjugated platinum(IV) complexes in the form Pt(L2)(A2)(OH)R based on the clinical drug cisplatin and oxaliplatin were designed, synthesized and evaluated as antitumor agents in vitro and in vivo. The conjugates possessing both pH and redox dual-responsive properties exhibited more potent cytotoxicity in seven different human cancer cell lines and lower toxicity to the normal 3T3 cells than cisplatin, oxaliplatin and even the reported bis-functionalized glycosylated platinum(IV) complexes indicating the enhanced safety of the sugar conjugates. Cellular drug uptake and DNA platination were also superior to cisplatin, oxaliplatin and the reported bis-functionalized ones. Peak current of B7 and B8 with the scan rate of 200mv/s at the concentration of 0.08 mM was 5-fold higher at pH 6.4 than the pH 7.4, indicating that carbohydrate-conjugated mono-functionalized platinum(IV) complexes possessed both pH and redox dual-responsive properties in the cancer cells. The in vivo assays demonstrated that the Pt(IV) compounds could inhibit the growth of MCF-7 tumour and exert more safety than oxaliplatin.

Keywords: Antitumor; Cancer; Glycosylation; Metal complexe; Platinum.

MeSH terms

  • Animals
  • Antineoplastic Agents / pharmacology*
  • Cell Survival / drug effects*
  • Cisplatin / pharmacology
  • Female
  • Flow Cytometry
  • Glycosylation
  • Humans
  • Hydrogen-Ion Concentration
  • In Vitro Techniques
  • Mice
  • Neoplasms / drug therapy
  • Neoplasms / pathology
  • Organoplatinum Compounds / chemistry*
  • Organoplatinum Compounds / pharmacology*
  • Oxaliplatin
  • Oxidation-Reduction
  • Tumor Cells, Cultured
  • Xenograft Model Antitumor Assays

Substances

  • Antineoplastic Agents
  • Organoplatinum Compounds
  • Oxaliplatin
  • Cisplatin