Structure and specificity of a permissive bacterial C-prenyltransferase

Nat Chem Biol. 2017 Apr;13(4):366-368. doi: 10.1038/nchembio.2285. Epub 2017 Feb 6.

Abstract

This study highlights the biochemical and structural characterization of the L-tryptophan C6 C-prenyltransferase (C-PT) PriB from Streptomyces sp. RM-5-8. PriB was found to be uniquely permissive to a diverse array of prenyl donors and acceptors including daptomycin. Two additional PTs also produced novel prenylated daptomycins with improved antibacterial activities over the parent drug.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Dimethylallyltranstransferase / chemistry*
  • Dimethylallyltranstransferase / metabolism*
  • Models, Molecular
  • Molecular Structure
  • Streptomyces / enzymology*
  • Substrate Specificity

Substances

  • Dimethylallyltranstransferase

Associated data

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