Evaluation of two novel chemiluminescence immunoassays for the detection of Clostridium difficile glutamate dehydrogenase and toxin A&B

J Microbiol Methods. 2017 Apr:135:63-65. doi: 10.1016/j.mimet.2017.02.004. Epub 2017 Feb 10.

Abstract

A novel immunoassay for Clostridium difficile glutamate dehydrogenase (GDH) and toxin A&B (LIAISON, DiaSorin) was compared to another GDH assay (Alere), PCR and toxigenic culture. The GDH-DiaSorin is slightly more sensitive than the GDH-Alere. Sensitivity of the Toxin-Diasorin test is in accordance to the sensitivity of other immunoassays in literature.

Keywords: Clostridium difficile; Glutamate dehydrogenase; Toxins A&B.

MeSH terms

  • Bacterial Proteins / analysis
  • Bacterial Proteins / genetics
  • Bacterial Proteins / isolation & purification*
  • Bacterial Toxins / genetics
  • Bacterial Toxins / isolation & purification*
  • Bacteriological Techniques / methods
  • Clostridioides difficile / enzymology*
  • Clostridioides difficile / genetics*
  • Enterotoxins / genetics
  • Enterotoxins / isolation & purification*
  • Feces / chemistry
  • Feces / microbiology
  • Glutamate Dehydrogenase / genetics
  • Glutamate Dehydrogenase / isolation & purification*
  • Humans
  • Immunoenzyme Techniques / instrumentation
  • Immunoenzyme Techniques / methods*
  • Luminescence*
  • Polymerase Chain Reaction / methods
  • Predictive Value of Tests
  • Sensitivity and Specificity

Substances

  • Bacterial Proteins
  • Bacterial Toxins
  • Enterotoxins
  • tcdA protein, Clostridium difficile
  • toxB protein, Clostridium difficile
  • Glutamate Dehydrogenase