Use of human methylation arrays for epigenome research in the common marmoset (Callithrix jacchus)

Junko Ueda; Yui Murata; Miki Bundo; Arata Oh-Nishi; Hidetoshi Kassai; Tempei Ikegame; Zhilei Zhao; Seiichiro Jinde; Atsu Aiba; Tetsuya Suhara; Kiyoto Kasai; Tadafumi Kato; Kazuya Iwamoto

doi:10.1016/j.neures.2017.02.005

Use of human methylation arrays for epigenome research in the common marmoset (Callithrix jacchus)

Neurosci Res. 2017 Jul:120:60-65. doi: 10.1016/j.neures.2017.02.005. Epub 2017 Feb 17.

Authors

Junko Ueda¹, Yui Murata², Miki Bundo³, Arata Oh-Nishi⁴, Hidetoshi Kassai⁵, Tempei Ikegame⁶, Zhilei Zhao⁶, Seiichiro Jinde⁶, Atsu Aiba⁵, Tetsuya Suhara⁴, Kiyoto Kasai⁶, Tadafumi Kato¹, Kazuya Iwamoto⁷

Affiliations

¹ Laboratory for Molecular Dynamics of Mental Disorders, RIKEN Brain Science Institute, 2-1 Hirosawa, Wako City, Saitama, 351-0198, Japan.
² Department of Neuropsychiatry, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan; Department of Molecular Brain Science, Graduate School of Life Sciences, Kumamoto University, 1-1-1 Honjo, Kumamoto City, Kumamoto, 860-8556, Japan.
³ Department of Molecular Brain Science, Graduate School of Life Sciences, Kumamoto University, 1-1-1 Honjo, Kumamoto City, Kumamoto, 860-8556, Japan.
⁴ Department of Functional Brain Imaging, National Institute of Radiological Sciences, National Institute for Quantum and Radiological Sciences and Technology, 4-9-1 Anagawa, Inage-ku, Chiba-shi, Chiba, 263-8555, Japan.
⁵ Laboratory of Animal Resources, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan.
⁶ Department of Neuropsychiatry, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan.
⁷ Department of Molecular Brain Science, Graduate School of Life Sciences, Kumamoto University, 1-1-1 Honjo, Kumamoto City, Kumamoto, 860-8556, Japan. Electronic address: iwamotok@kumamoto-u.ac.jp.

PMID: 28215819
DOI: 10.1016/j.neures.2017.02.005

Abstract

We examined the usefulness of commercially available DNA methylation arrays designed for the human genome (Illumina HumanMethylation450 and MethylationEPIC) for high-throughput epigenome analysis of the common marmoset, a nonhuman primate suitable for research on neuropsychiatric disorders. From among the probes on the methylation arrays, we selected those available for the common marmoset. DNA methylation data were obtained from genomic DNA extracted from the frontal cortex and blood samples of adult common marmosets as well as the frontal cortex of neonatal marmosets. About 10% of the probes on the arrays were estimated to be useful for DNA methylation assay in the common marmoset. Strong correlations existed between human and marmoset DNA methylation data. Illumina methylation arrays are useful for epigenome research using the common marmoset.

Keywords: Beadarray; Cytosine modification; DNA methylation; Epigenetics; Nonhuman primate.

MeSH terms

Animals
Callithrix
DNA Methylation*
Epigenesis, Genetic*
Epigenomics / instrumentation
Epigenomics / methods*
Humans
Male
Species Specificity