Development of a real-time PCR assay for the direct detection of Candida species causing Vulvovaginal candidiasis

Diagn Microbiol Infect Dis. 2017 May;88(1):39-40. doi: 10.1016/j.diagmicrobio.2017.01.012. Epub 2017 Jan 25.

Abstract

Identification of Candida species by traditional methods can be time-consuming and have limited analytical sensitivity. We developed a multiplex real-time PCR assay for detection and differentiation of Candida species causing vulvovaginal candidiasis (VVC). Overall, this PCR assay is a powerful diagnostic tool offering superior accuracy, sensitivity, and specificity.

Keywords: Assay development; Candidiasis; Diagnostic; PCR; Vulvovaginal.

MeSH terms

  • Candida / genetics
  • Candida / isolation & purification*
  • Candidiasis, Vulvovaginal / diagnosis*
  • Candidiasis, Vulvovaginal / microbiology*
  • Female
  • Humans
  • Molecular Diagnostic Techniques / methods*
  • Multiplex Polymerase Chain Reaction / methods*
  • Real-Time Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity