Immunohistochemical response in rats of beta-tricalcium phosphate (TCP) with or without BMP-2 in the production of collagen matrix critical defects

Acta Histochem. 2017 Apr;119(3):302-308. doi: 10.1016/j.acthis.2017.02.006. Epub 2017 Mar 3.

Abstract

This study aimed to assess the biological response of BMP-2 (bone morphogenetic protein-2) in supplementation with β-tricalcium phosphate (TCP) as a carrier in the bone healing of surgical defects in rats' calvaria. A critical-size defect (5mm in diameter) was filled with β-TCP alone or added with that plus 5mg of BMP-2 at 5, 15, and 30 postoperative days. Histomorphometric and immunohistochemical (osteocalcin, collagen type I, and metalloproteinase-9) analysis was performed to assess the features of bone healing. Histological behavior and collagen type I labeling showed increased formation of the collagen matrix, leading to a higher percentage of newly formed bone and biomaterial for tissue and more total mineralization of pure TCP when compared to the other groups. The supplementation with BMP-2 promoted faster TCP remodeling; however, there was no statistically significant difference for the bone formed in both groups (P>0.05). Collagen-matrix formation and new bone formation reached maximum levels when the defects were filled with pure TCP, even exceeding the levels from BMP-2 supplementation.

Keywords: BMP; Collagen matrix; Histologic evaluation; Immunohistochemistry.

MeSH terms

  • Animals
  • Bone Morphogenetic Protein 2 / pharmacology*
  • Bone and Bones / drug effects
  • Calcium Phosphates / pharmacology*
  • Collagen / metabolism*
  • Immunohistochemistry
  • Male
  • Rats
  • Rats, Wistar
  • Skull / drug effects
  • Skull / injuries*
  • Skull / surgery
  • Wound Healing / drug effects*

Substances

  • Bone Morphogenetic Protein 2
  • Calcium Phosphates
  • beta-tricalcium phosphate
  • Collagen