A novel technique for tumor localization and targeted lymphatic mapping in early-stage lung cancer

J Thorac Cardiovasc Surg. 2017 Sep;154(3):1110-1118. doi: 10.1016/j.jtcvs.2016.12.058. Epub 2017 Feb 10.

Abstract

Objective: To investigate safety and feasibility of navigational bronchoscopy (NB)-guided near-infrared (NIR) localization of small, ill-defined lung lesions and sentinel lymph nodes (SLN) for accurate staging in patients with non-small cell lung cancer (NSCLC).

Methods: Patients with known or suspected stage I NSCLC were enrolled in a prospective pilot trial for lesion localization and SLN mapping via NB-guided NIR marking. Successful localization, SLN detection rates, histopathologic status of SLN versus overall nodes, and concordance to initial clinical stage were measured. Ex vivo confirmation of NIR+ SLNs and adverse events were recorded.

Results: Twelve patients underwent NB-guided marking with indocyanine green of lung lesions ranging in size from 0.4 to 2.2 cm and located 0.1 to 3 cm from the pleural surface. An NIR+ "tattoo" was identified in all cases. Ten patients were diagnosed with NSCLC and 9 SLNs were identified in 8 of the 10 patients, resulting in an 80% SLN detection rate. SLN pathologic status was 100% sensitive and specific for overall nodal status with no false-negative results. Despite previous nodal sampling, one patient was found to have metastatic disease in the SLN alone, a 12.5% rate of disease upstaging with NIR SLN mapping. SLN were detectable for up to 3 hours, allowing time for obtaining a tissue diagnosis and surgical resection. There were no adverse events associated with NB-labeling or indocyanine green dye itself.

Conclusions: NB-guided NIR lesion localization and SLN identification was safe and feasible. This minimally invasive image-guided technique may permit the accurate localization and nodal staging of early stage lung cancers.

Keywords: lung cancer; navigational bronchoscopy; near-infrared; sentinel lymph node; tumor localization.

Publication types

  • Clinical Trial

MeSH terms

  • Bronchoscopy
  • Carcinoma, Non-Small-Cell Lung / diagnostic imaging*
  • Carcinoma, Non-Small-Cell Lung / surgery
  • Coloring Agents
  • Feasibility Studies
  • Female
  • Humans
  • Indocyanine Green*
  • Lung Neoplasms / diagnostic imaging*
  • Lung Neoplasms / surgery
  • Lymph Nodes / diagnostic imaging*
  • Lymph Nodes / pathology
  • Lymph Nodes / surgery
  • Lymphatic Metastasis
  • Male
  • Middle Aged
  • Pilot Projects
  • Prospective Studies
  • Sensitivity and Specificity
  • Sentinel Lymph Node Biopsy
  • Spectroscopy, Near-Infrared*
  • Thoracic Surgery, Video-Assisted

Substances

  • Coloring Agents
  • Indocyanine Green