Current methodologies on genotyping for nosocomial pathogen methicillin-resistant Staphylococcus aureus (MRSA)

Jian Miao; Lequn Chen; Jingwen Wang; Wenxin Wang; Dingqiang Chen; Lin Li; Bing Li; Yang Deng; Zhenbo Xu

doi:10.1016/j.micpath.2017.03.010

Current methodologies on genotyping for nosocomial pathogen methicillin-resistant Staphylococcus aureus (MRSA)

Microb Pathog. 2017 Jun:107:17-28. doi: 10.1016/j.micpath.2017.03.010. Epub 2017 Mar 9.

Authors

Jian Miao¹, Lequn Chen¹, Jingwen Wang¹, Wenxin Wang¹, Dingqiang Chen², Lin Li³, Bing Li³, Yang Deng⁴, Zhenbo Xu⁵

Affiliations

¹ School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, PR China.
² Department of Laboratory Medicine, First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, PR China.
³ School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, PR China; Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Guangzhou 510640, PR China.
⁴ School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, PR China; State Key Laboratory of Biological Fermentation Engineering of Beer, Tsingtao Brewery Co. Ltd., Qingdao 266061, PR China. Electronic address: dengyangscut@163.com.
⁵ School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, PR China; Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Guangzhou 510640, PR China; Department of Microbial Pathogenesis, University of Maryland, Baltimore 21201, USA. Electronic address: zhenbo.xu@hotmail.com.

PMID: 28284852
DOI: 10.1016/j.micpath.2017.03.010

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is a common pathogen in hospitals and the community. As the rapid spread and wide distribution of antimicrobial resistance (such as MRSA), treatment for infectious diseases caused by microorganisms has become a vital threat. Thus, early identification and genotyping are essential for further therapeutic treatment and the control of rapid expansion of MRSA. In combination with applications and data feedbacks, this review focused on the currently available molecular-based assays on their utility and performance for rapid typing of MRSA, especially on effective molecular-based methods. Besides, a common mobile element SCCmec and prevalence of HA-MRSA, LA-MRSA and CA-MRSA were introduced in this review in order to provide a more complete profile of MRSA.

Keywords: MRSA; Molecular methods; SCCmec; Typing.

Publication types

Review

MeSH terms

Amplified Fragment Length Polymorphism Analysis / methods
Antigens, Bacterial / genetics
Bacterial Proteins / genetics
Bacterial Typing Techniques / instrumentation
Bacterial Typing Techniques / methods*
Coagulase / genetics
Cross Infection / diagnosis
Cross Infection / microbiology*
Electrophoresis, Gel, Pulsed-Field / methods
Genotype*
Genotyping Techniques / instrumentation
Genotyping Techniques / methods*
Humans
Methicillin-Resistant Staphylococcus aureus / classification
Methicillin-Resistant Staphylococcus aureus / genetics*
Methicillin-Resistant Staphylococcus aureus / isolation & purification*
Multilocus Sequence Typing / methods
Oligonucleotide Array Sequence Analysis / methods
Penicillin-Binding Proteins / genetics
Polymorphism, Genetic
Polymorphism, Restriction Fragment Length
Random Amplified Polymorphic DNA Technique / methods
Staphylococcal Infections / diagnosis
Staphylococcal Infections / microbiology*
Trans-Activators / genetics

Substances

Agr protein, Staphylococcus aureus
Antigens, Bacterial
Bacterial Proteins
Coagulase
Penicillin-Binding Proteins
Trans-Activators
mecA protein, Staphylococcus aureus
streptococcal protective antigen