(Ca2+ + Mg2+)-dependent ATPase mRNA from smooth muscle sarcoplasmic reticulum differs from that in cardiac and fast skeletal muscles

FEBS Lett. 1988 Feb 29;229(1):45-8. doi: 10.1016/0014-5793(88)80794-4.

Abstract

We have investigated some characteristics of the sarcoplasmic reticulum (Ca2+ + Mg2+)-dependent ATPase (Ca2+-ATPase) mRNA from smooth muscle using specific cDNA probes isolated from a rat heart cDNA library. RNA blot analysis has shown that the Ca2+-ATPase mRNA expressed in smooth muscle is identical in size to the cardiac mRNA but differs from that of fast skeletal muscle. S1 nuclease mapping has moreover shown that the cardiac and smooth muscle isoforms possess different 3'-end sequences. These results indicate that a distinct sarcoplasmic reticulum Ca2+-ATPase mRNA is present in smooth muscle.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Ca(2+) Mg(2+)-ATPase / genetics*
  • Calcium-Transporting ATPases / genetics*
  • DNA / analysis
  • Electrophoresis, Polyacrylamide Gel
  • Endonucleases / metabolism
  • Isoenzymes / genetics*
  • Muscle, Smooth / enzymology*
  • Muscle, Smooth / ultrastructure
  • Muscles / enzymology*
  • Myocardium / enzymology*
  • RNA, Messenger / analysis*
  • Rats
  • Sarcoplasmic Reticulum / enzymology*
  • Single-Strand Specific DNA and RNA Endonucleases

Substances

  • Isoenzymes
  • RNA, Messenger
  • DNA
  • Endonucleases
  • Single-Strand Specific DNA and RNA Endonucleases
  • Ca(2+) Mg(2+)-ATPase
  • Calcium-Transporting ATPases