An in vitro bacterial surface migration assay underneath sterile barrier material commonly found in a hospital setting

J Perinatol. 2017 Jul;37(7):848-852. doi: 10.1038/jp.2017.28. Epub 2017 Mar 23.

Abstract

Objective: To determine what barrier material used in hospital neonatal intensive care units most effectively blocks bacterial migration.

Study design: Bacterial migration distance was compared across simple and complex solid media using Escherichia coli, an early and common neonatal gut colonizer, and Staphylococcus aureus, a common skin bacterium, across polystyrene, medical-grade silicone, hydrocolloid dressing and transparent film dressing as barrier materials on complex solid media.

Results: Bacterial migration was significantly greater on complex versus simple solid media. Bacteria migrated farthest beneath hydrocolloid dressing and transparent film dressing, while migration underneath polystyrene and medical-grade silicone was generally comparable to no barrier.

Conclusions: Commonly used hydrocolloid dressing and transparent film dressing surprisingly increases bacterial migration, possibly by providing a wet capillary surface for bacteria to attach to or inducing biofilm formation. Using polystyrene or silicone to interface with the site of catheter insertion may best avoid a bacterial wicking phenomenon.

MeSH terms

  • Bacterial Adhesion
  • Bandages, Hydrocolloid*
  • Escherichia coli / physiology*
  • Hospitals
  • Humans
  • Polystyrenes*
  • Silicones*
  • Staphylococcus aureus / physiology*
  • Surface Properties
  • Time Factors

Substances

  • Polystyrenes
  • Silicones