Controlling transmembrane protein concentration and orientation in supported lipid bilayers

Chem Commun (Camb). 2017 Apr 11;53(30):4250-4253. doi: 10.1039/c7cc01023k.

Abstract

The trans-membrane protein - proteorhodopsin (pR) has been incorporated into supported lipid bilayers (SLB). In-plane electric fields have been used to manipulate the orientation and concentration of these proteins, within the SLB, through electrophoresis leading to a 25-fold increase concentration of pR.

MeSH terms

  • Electrophoresis
  • Lipid Bilayers / chemistry*
  • Microscopy, Atomic Force
  • Rhodopsins, Microbial / analysis*

Substances

  • Lipid Bilayers
  • Rhodopsins, Microbial
  • proteorhodopsin