Abstract
Relapse and metastasis are frequent in colon cancer and may be linked to stem cell characteristics. This study isolated side population (SP) cells from a colon cancer cell line (Colo-320) and examined their self-renewal and differentiation abilities. Compared to non-SP (NSP) cells, SP colon cancer cells were more tumorigenic in vivo and exhibited more invasive characteristics and a greater ability to form colonies. Additionally, more cells were in G0/G1 phase and more highly expressed the multidrug resistance protein BCRP/ABCG2. We achieved enhanced chemotherapy sensitivity by transfecting SP cells with a hairpin-like, small interfering RNA (siRNA) eukaryotic expression plasmid targeting BCRP/ABCG2.
Keywords:
RNAi; bcrp/abcg2; chemotherapy sensitivity; colon cancer; side population cells.
MeSH terms
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ATP Binding Cassette Transporter, Subfamily G, Member 2 / antagonists & inhibitors*
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ATP Binding Cassette Transporter, Subfamily G, Member 2 / genetics
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Animals
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Cell Differentiation / drug effects
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Cell Line, Tumor
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Cell Proliferation / drug effects
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Colonic Neoplasms / genetics
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Colonic Neoplasms / metabolism
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Colonic Neoplasms / therapy*
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Drug Synergism
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Drug Therapy
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Fluorouracil / administration & dosage*
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Fluorouracil / pharmacology
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Gene Expression Regulation, Neoplastic / drug effects
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Humans
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Mice
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Neoplasm Proteins / antagonists & inhibitors*
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Neoplasm Proteins / genetics
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Neoplastic Stem Cells / drug effects*
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Neoplastic Stem Cells / pathology
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RNA, Small Interfering / pharmacology*
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Side-Population Cells / drug effects*
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Side-Population Cells / pathology
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Xenograft Model Antitumor Assays
Substances
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ABCG2 protein, human
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ATP Binding Cassette Transporter, Subfamily G, Member 2
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Neoplasm Proteins
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RNA, Small Interfering
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Fluorouracil