Transfection of Primary Human Skin Fibroblasts for Peroxisomal Studies

Janet Koster; Hans R Waterham

doi:10.1007/978-1-4939-6937-1_7

Transfection of Primary Human Skin Fibroblasts for Peroxisomal Studies

Methods Mol Biol. 2017:1595:63-67. doi: 10.1007/978-1-4939-6937-1_7.

Authors

Janet Koster¹, Hans R Waterham²

Affiliations

¹ Laboratory Genetic Metabolic Diseases, Department of Clinical Chemistry, Academic Medical Center, University of Amsterdam, Meibergdreef 9, 1105 AZ, Amsterdam, The Netherlands.
² Laboratory Genetic Metabolic Diseases, Department of Clinical Chemistry, Academic Medical Center, University of Amsterdam, Meibergdreef 9, 1105 AZ, Amsterdam, The Netherlands. h.r.waterham@amc.uva.nl.

PMID: 28409452
DOI: 10.1007/978-1-4939-6937-1_7

Abstract

Functional studies with primary human skin fibroblasts from patients with a peroxisomal disorder often require efficient transfection with plasmids to correct the genetic defect or to express heterologous reporter proteins. Here, we describe a protocol we commonly use for efficient nonviral transfection of primary human skin fibroblasts.

Keywords: Functional complementation; Nucleofection; Primary cells; Transfection.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Fibroblasts / metabolism*
Humans
Peroxisomes / metabolism*
Primary Cell Culture
Skin / cytology*
Transfection*