Correlation between gyrA and CmeR Box Polymorphism and Fluoroquinolone Resistance in Campylobacter jejuni Isolates in China

Antimicrob Agents Chemother. 2017 Jun 27;61(7):e00422-17. doi: 10.1128/AAC.00422-17. Print 2017 Jul.

Abstract

Sequence analysis of 79 ciprofloxacin-resistant Campylobacter jejuni isolates collected in China showed resistance-related sequence variations in gyrA and CmeR-Box. All the isolates contain an identical Thr-86-Ile substitution in GyrA. Several novel CmeR-Box variations, including point substitutions, deletion, and insertion, were identified. The point insertion or deletion led to dramatically reduced binding of CmeR to the cmeABC promoter, which significantly increases the expression of cmeABC and contributes to the high fluoroquinolone resistance.

Keywords: Campylobacter jejuni; DNA gyrase; cmeABC; fluoroquinolone resistance.

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Bacterial Proteins / genetics*
  • Campylobacter jejuni / drug effects*
  • Campylobacter jejuni / genetics*
  • Campylobacter jejuni / pathogenicity
  • DNA Gyrase / genetics*
  • Drug Resistance, Bacterial / genetics
  • Drug Resistance, Multiple, Bacterial / genetics
  • Fluoroquinolones / pharmacology*
  • Microbial Sensitivity Tests
  • Mutation
  • Polymorphism, Genetic / genetics

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • Fluoroquinolones
  • DNA Gyrase