Two alpha-adrenergic contractions with different Ca2+ activation mechanisms in response to field nerve stimulation in the circular smooth muscle of guinea-pig vas deferens

J Auton Pharmacol. 1988 Jun;8(2):119-25. doi: 10.1111/j.1474-8673.1988.tb00175.x.

Abstract

1. Two noradrenergic components of contraction were induced by field nerve stimulation of the circular smooth muscle of guinea-pig vas deferens. One component occurred during the stimulation (former-response) and the other component occurred after stimulation had ceased (after-response), with a distinct tension fall between these two responses. 2. The effects of stimulation frequency and duration upon these components were examined. 3. Prazosin (0.05-0.5 microM) suppressed both responses, whereas nifedipine (0.02 microM) suppressed only the after-response. 4. It is suggested that in this tissue there are two neurogenic alpha-adrenergic contractions which possess different Ca2+ activation mechanisms. The former-response is due to an increase in cytoplasmic Ca2+ concentration through a pathway independent of action potentials, while the after-response is attributed to action potentials arising as a secondary response to the released noradrenaline.

MeSH terms

  • Animals
  • Calcium / physiology*
  • Electric Stimulation
  • Guinea Pigs
  • In Vitro Techniques
  • Male
  • Muscle Contraction* / drug effects
  • Nifedipine / pharmacology
  • Prazosin / pharmacology
  • Receptors, Adrenergic, alpha / physiology*
  • Vas Deferens / drug effects
  • Vas Deferens / physiology*

Substances

  • Receptors, Adrenergic, alpha
  • Nifedipine
  • Calcium
  • Prazosin