Transamination of LTE4 by cysteine conjugate aminotransferase

Biochem Biophys Res Commun. 1988 Sep 30;155(3):1119-25. doi: 10.1016/s0006-291x(88)81256-7.

Abstract

Leukotriene E4 (LTE4) was transaminated to 5(S)-hydroxy-6(R)-S-(2-keto-3-thiopropionyl)-7,9-trans-11,14-cis- eicosatetraenoic acid (tentatively designated as LTG4) by cysteine conjugate aminotransferase I purified from rat liver supernatant in the presence of alpha-ketoglutaric acid or alpha-keto-gamma-methiolbutyric acid. The transamination activity was present in the kidney as well as in the liver, but not in the lung or leukocytes.

MeSH terms

  • Animals
  • Ketoglutaric Acids / metabolism
  • Kidney / enzymology
  • Leukotriene E4
  • Leukotrienes / metabolism
  • Liver / enzymology
  • Male
  • Mass Spectrometry
  • Methionine / analogs & derivatives
  • Methionine / metabolism
  • Rats
  • Rats, Inbred Strains
  • SRS-A / analogs & derivatives*
  • SRS-A / metabolism
  • Transaminases / metabolism*

Substances

  • Ketoglutaric Acids
  • Leukotrienes
  • SRS-A
  • 5-hydroxy-6-(2-keto-3-thiopropionyl)-7,9,11,14-eicosatetraenoic acid
  • 2-keto-4-methylthiobutyric acid
  • Leukotriene E4
  • Methionine
  • Transaminases
  • cysteine aminotransferase