Objective: To investigate the effects of mandibular advancement device (MAD) upon nuclear factor κB (NF-κB), tumor necrosis factor α (TNF-α) and interleukin 6 (IL-6) in the genioglossus. Methods: Eighteen New Zealand white rabbits (male, six months old), in accordance with the random number table, were equally divided into three groups, the control group, obstructive sleep apnea hypopnea syndrome (OSAHS) group and MAD group. All animals were induced to sleep in supine position for 2 hours every morning in the next 8 weeks. The specimens of genioglossus were prepared. The relative expression of NF-κB p65 was measured with Western blotting and the mass concentration of TNF-α and IL-6 was determined with enzyme-linked immunosorbent assay. Results: The relative expressions of NF-κB p65 protein in genioglossus in the control group, OSAHS group and MAD group were 0.24±0.07, 0.44±0.08 and 0.30±0.09, respectively. The mass concentrations of TNF-α in genioglossus in the control group, OSAHS group and MAD group were (0.065±0.020), (0.097±0.018) and (0.071±0.020) μg/L, respectively. The mass concentrations of IL-6 in genioglossus in the control group, OSAHS group and MAD group were (0.063±0.013), (0.093±0.017), and (0.069±0.014) μg/L, respectively. For the above indicators, the data in OSAHS group were all significantly higher than that in MAD group and the control group (P<0.05). No significant difference was found between MAD group and the control group (P>0.05). Conclusions: Treatment of OSAHS with MAD decreased the mass concentration of TNF-α and IL-6 leading to fatigue of genioglossus, reduced the activation of NF-κB and played a significant role in protecting genioglossus.
目的： 研究下颌前移矫治器（mandibular advancement device，MAD）治疗对阻塞性睡眠呼吸暂停低通气综合征（obstructive sleep apnea hypopnea syndrome，OSAHS）兔颏舌肌核因子κB（nuclear factor κB，NF-κB）、肿瘤坏死因子α（tumor necrosis factor α，TNF-α）、白细胞介素6（interleukin 6，IL-6）表达的影响，探讨炎性机制在OSAHS致颏舌肌病变中的作用。 方法： 通过随机数字表将18只6个月龄雄性新西兰大白兔均分为3组，即对照组、OSAHS组和MAD组。每日上午诱导动物仰卧位睡眠2 h，持续8周。制备颏舌肌标本，蛋白质印迹法测定细胞核NF-κB p65蛋白相对表达量，酶联免疫吸附测定法检测TNF-α、IL-6质量浓度。 结果： 对照组、OSAHS组和MAD组颏舌肌NF-κB p65蛋白相对表达量分别为0.24±0.07、0.44±0.08、0.30±0.09；TNF-α质量浓度分别为（0.065±0.020）、（0.097±0.018）和（0.071±0.020）μg/L；IL-6质量浓度分别为（0.063±0.013）、（0.093±0.017）和（0.069±0.014）μg/L。OSAHS组NF-κB p65蛋白及TNF-α、IL-6均显著高于对照组和MAD组（P<0.05），对照组和MAD组NF-κB p65蛋白、TNF-α、IL-6差异均无统计学意义（P>0.05）。 结论： MAD治疗OSAHS可降低导致颏舌肌疲劳性增加的TNF-α、IL-6质量浓度，减少NF-κB活化，对颏舌肌起保护作用。.
Keywords: Mandibular advancement device; NF-kappa B; Obstructive sleep apnoea hypopnea syndrome; Tumor necrosis factor-alpha.