Abstract
In order to comprehend the function of a particular protein, identification of the interacting protein partners is a useful approach. Co-immunoprecipitation (Co-IP) is employed to test physical interactions between proteins. Specific antibodies or antibodies against tagged versions can be used to immunoprecipitate the proteins. In this chapter, we describe a method to carry out Co-IP using recombinant membrane proteins expressed in yeast microsomal fractions.
Keywords:
Antibody; Co-immunoprecipitation; Protein interaction; Protein–protein interaction; Yeast.
MeSH terms
-
Antibodies / chemistry*
-
Blotting, Western
-
Electrophoresis, Polyacrylamide Gel
-
Gene Expression
-
Immunoprecipitation / methods*
-
Isoenzymes / genetics
-
Isoenzymes / isolation & purification
-
Isoenzymes / metabolism
-
Ligands
-
Microsomes / chemistry
-
Protein Binding
-
Protein Interaction Mapping / methods*
-
Protein Kinase C / genetics
-
Protein Kinase C / isolation & purification*
-
Protein Kinase C / metabolism
-
Recombinant Proteins / genetics
-
Recombinant Proteins / isolation & purification
-
Recombinant Proteins / metabolism
-
Saccharomyces cerevisiae / chemistry
-
Saccharomyces cerevisiae / genetics
-
Saccharomyces cerevisiae / metabolism
-
Solanum lycopersicum / enzymology
-
Solanum lycopersicum / genetics*
Substances
-
Antibodies
-
Isoenzymes
-
Ligands
-
Recombinant Proteins
-
protein kinase N
-
Protein Kinase C