RutA-Catalyzed Oxidative Cleavage of the Uracil Amide Involves Formation of a Flavin-N5-oxide

Biochemistry. 2017 Jul 25;56(29):3708-3709. doi: 10.1021/acs.biochem.7b00493. Epub 2017 Jul 10.

Abstract

RutA is a novel flavoenzyme on the uracil catabolic pathway that catalyzes uracil ring opening by a unique amide oxidation reaction. Here we provide evidence that this reaction also involves the formation of a flavin-N5-oxide.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Catalysis
  • Dinitrocresols / chemistry*
  • Models, Chemical*
  • Polyenes / chemistry
  • Uracil / chemistry*

Substances

  • Dinitrocresols
  • Polyenes
  • 4,6-dinitro-o-cresol
  • Uracil
  • flavopentin