An integration-free, virus-free rhesus macaque induced pluripotent stem cell line (riPSC90) from embryonic fibroblasts

Enrique Sosa; Rachel Kim; Ernesto J Rojas; Linzi Hosohama; Jon D Hennebold; Kyle E Orwig; Amander T Clark

doi:10.1016/j.scr.2017.03.011

An integration-free, virus-free rhesus macaque induced pluripotent stem cell line (riPSC90) from embryonic fibroblasts

Stem Cell Res. 2017 May:21:5-8. doi: 10.1016/j.scr.2017.03.011. Epub 2017 Mar 15.

Authors

Enrique Sosa¹, Rachel Kim¹, Ernesto J Rojas¹, Linzi Hosohama¹, Jon D Hennebold², Kyle E Orwig³, Amander T Clark⁴

Affiliations

¹ Department of Molecular, Cell and Developmental Biology, Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA 90095, USA.
² Division of Reproductive & Developmental Sciences, Oregon National Primate Research Center, Beaverton, Oregon Department of Obstetrics and Gynecology, Oregon Health & Science University, Portland, OR 97006, USA.
³ Department of Obstetrics, Gynecology and Reproductive Sciences, Magee-Womans Research Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA 15213, USA.
⁴ Department of Molecular, Cell and Developmental Biology, Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA 90095, USA. Electronic address: clarka@ucla.edu.

Abstract

The rhesus macaque induced pluripotent stem cell (riPSC) line, UCLAi090-A (riPSC90), was generated from rhesus embryonic fibroblast (REF) cells called REF90. REF90 cells and the riPSC90 line were authenticated by short tandem repeat analysis and had a normal male (42, XY) karyotype. The riPSC90 line expressed markers of self-renewal including OCT4, NANOG, TRA-1-81 and SSEA4, and generated teratomas after transplantation into immunocompromised mice. riPSC90 could be used in parallel with riPSC89, which was derived from REFs cultured from a different rhesus macaque embryo (Sosa et al. 2016).

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Animals
Cell Line
Embryo, Mammalian / cytology
Embryo, Mammalian / metabolism*
Gene Transfer Techniques*
Induced Pluripotent Stem Cells / cytology
Induced Pluripotent Stem Cells / metabolism*
Macaca mulatta
Male
Mice
Mice, SCID
Transcription Factors* / biosynthesis
Transcription Factors* / genetics

Substances

Transcription Factors

Abstract

Publication types

MeSH terms

Substances

Grants and funding