First Complete Genomic Sequence of a Rabies Virus from the Republic of Tajikistan Obtained Directly from a Flinders Technology Associates Card

H Goharriz; D A Marston; F Sharifzoda; R J Ellis; D L Horton; T Khakimov; A Whatmore; K Khamroev; A N Makhmadshoev; M Bazarov; A R Fooks; A C Banyard

doi:10.1128/genomeA.00515-17

First Complete Genomic Sequence of a Rabies Virus from the Republic of Tajikistan Obtained Directly from a Flinders Technology Associates Card

Genome Announc. 2017 Jul 6;5(27):e00515-17. doi: 10.1128/genomeA.00515-17.

Authors

H Goharriz¹, D A Marston¹, F Sharifzoda, R J Ellis², D L Horton³, T Khakimov⁴, A Whatmore⁵, K Khamroev⁴, A N Makhmadshoev⁴, M Bazarov⁴, A R Fooks^{1

6}, A C Banyard⁷

Affiliations

¹ Wildlife Zoonoses & Vector-Borne Diseases Research Group, Animal and Plant Health Agency, Surrey, United Kingdom.
² Specialist Scientific Support Department, Animal and Plant Health Agency, Surrey, United Kingdom.
³ School of Veterinary Medicine, University of Surrey, Guildford, United Kingdom.
⁴ National Center for Veterinary Diagnostics, Dushanbe, Republic of Tajikistan.
⁵ Bacterial Characterization Team, Animal and Plant Health Agency, Surrey, United Kingdom.
⁶ Institute for Infection and Immunity, St. George's Hospital Medical School, University of London, London, United Kingdom.
⁷ Wildlife Zoonoses & Vector-Borne Diseases Research Group, Animal and Plant Health Agency, Surrey, United Kingdom Ashley.Banyard@apha.gsi.gov.uk.

Abstract

A brain homogenate derived from a rabid dog in the district of Tojikobod, Republic of Tajikistan, was applied to a Flinders Technology Associates (FTA) card. A full-genome sequence of rabies virus (RABV) was generated from the FTA card directly without extraction, demonstrating the utility of these cards for readily obtaining genetic data.