Abstract
Chromatin looping is key to gene regulation, yet no broadly applicable methods to selectively modify chromatin loops have been described. We have engineered a method for chromatin loop reorganization using CRISPR-dCas9 (CLOuD9) to selectively and reversibly establish chromatin loops. We demonstrate the power of this technology to selectively modulate gene expression at targeted loci.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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CRISPR-Cas Systems*
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Chromatin Assembly and Disassembly*
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DEAD-box RNA Helicases / metabolism
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HEK293 Cells
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Humans
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K562 Cells
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Promoter Regions, Genetic
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beta-Globins / genetics
Substances
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beta-Globins
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DDX17 protein, human
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Ddx5 protein, human
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DEAD-box RNA Helicases