Murine bone marrow-derived DCs activated by porcine rotavirus stimulate the Th1 subtype response in vitro

Microb Pathog. 2017 Sep:110:325-334. doi: 10.1016/j.micpath.2017.07.015. Epub 2017 Jul 12.

Abstract

Rotavirus (RV) infection causes acute, watery dehydrating diarrhea and even death in infants and other young animals, resulting in a severe economic burden; however, little is known about the innate immune mechanisms associated with RV infection. Dendritic cells (DCs), which are professional antigen-presenting cells (APCs), serve as a bridge connecting the innate and adaptive immune system. In this study, the interaction between murine bone marrow-derived DCs (BMDCs) and porcine rotavirus (PRV) was investigated in vitro. Upon stimulation, the expression levels of MHC-II, CD40, CD80, CD86 and CD83 in BMDCs increased in a time-dependent manner, indicating activation and maturation by PRV. In addition, up-regulated Toll-like receptor 2 (TLR2), TLR3 and NF-κB increased the production of interleukin-12 and interferon-γ. The PRV-stimulated BMDCs also showed increased stimulatory capacity in mixed lymphocyte reactions and promoted the Th1 subtype response.

Keywords: Activation; Bone marrow-derived DCs (BMDCs); Rotavirus.

MeSH terms

  • Adaptive Immunity
  • Animals
  • Antigen-Presenting Cells / immunology
  • Antigen-Presenting Cells / virology
  • Antigens, CD / metabolism
  • B7-1 Antigen / metabolism
  • B7-2 Antigen / metabolism
  • Bone Marrow / immunology*
  • CD40 Antigens / metabolism
  • CD83 Antigen
  • Cytokines / genetics
  • Cytokines / metabolism
  • Dendritic Cells / cytology
  • Dendritic Cells / immunology*
  • Dendritic Cells / metabolism
  • Dendritic Cells / virology*
  • Gene Expression Regulation
  • Genes, MHC Class II
  • Host-Pathogen Interactions / immunology
  • Immunity, Innate
  • Immunity, Mucosal
  • Immunoglobulins / metabolism
  • Interferon-gamma / metabolism
  • Interleukin-12 / metabolism
  • Male
  • Membrane Glycoproteins / metabolism
  • Mice
  • Mice, Inbred BALB C
  • NF-kappa B / metabolism
  • Rotavirus / immunology*
  • Rotavirus / pathogenicity
  • Rotavirus Infections / immunology*
  • Swine
  • Th1 Cells / immunology*
  • Toll-Like Receptor 2 / genetics
  • Toll-Like Receptor 2 / metabolism
  • Toll-Like Receptor 3 / genetics
  • Toll-Like Receptor 3 / metabolism
  • Up-Regulation

Substances

  • Antigens, CD
  • B7-1 Antigen
  • B7-2 Antigen
  • CD40 Antigens
  • Cytokines
  • Immunoglobulins
  • Membrane Glycoproteins
  • NF-kappa B
  • TLR3 protein, mouse
  • Toll-Like Receptor 2
  • Toll-Like Receptor 3
  • Interleukin-12
  • Interferon-gamma