Genetic Interaction Screens in Schizosaccharomyces pombe Using the Pombe Epistasis Mapper (PEM) System and a Manual Colony Replicator

Cold Spring Harb Protoc. 2018 Feb 1;2018(2). doi: 10.1101/pdb.prot091967.

Abstract

In laboratories in which a colony-replicating robot is not available, manual replication provides a good, low-cost alternative for genetic interaction screening using the Pombe Epistasis Mapper (PEM) system. The protocol presented here describes the minimum number of steps required to identify genetic interactions. First, a query deletion is introduced to a library of deletion mutants by mating. Through a series of subsequent selection steps, single and double mutants are isolated and analyzed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromosome Mapping / instrumentation*
  • Chromosome Mapping / methods*
  • Epistasis, Genetic*
  • Genes, Fungal*
  • Genetics, Microbial / instrumentation*
  • Genetics, Microbial / methods*
  • Mutation
  • Schizosaccharomyces / genetics*
  • Selection, Genetic