Legionella pneumophila LPS to evaluate urinary antigen tests

Diagn Microbiol Infect Dis. 2017 Oct;89(2):89-91. doi: 10.1016/j.diagmicrobio.2017.06.013. Epub 2017 Jun 23.

Abstract

Three urinary antigen tests were compared using purified Legionella pneumophila (Lp) LPS. For Lp serogroup1, Sofia®FIA and Binax®EIA limits of detection (LOD) were similar; that of BinaxNOW® lower. For all tests the LOD was higher with LPS from non-Pontiac compared to Pontiac-strains. The LOD was variable for other Lp serogroups.

Keywords: LPS; Legionella pneumophila; Pontiac; Urinary antigen; mAb3/1.

Publication types

  • Comparative Study

MeSH terms

  • Antigens, Bacterial / urine*
  • Fluorescent Antibody Technique / methods
  • Humans
  • Immunoenzyme Techniques / methods
  • Legionella pneumophila / immunology*
  • Legionella pneumophila / metabolism
  • Legionnaires' Disease / diagnosis*
  • Legionnaires' Disease / microbiology
  • Limit of Detection
  • Lipopolysaccharides / immunology*
  • Reagent Kits, Diagnostic
  • Sensitivity and Specificity

Substances

  • Antigens, Bacterial
  • Lipopolysaccharides
  • Reagent Kits, Diagnostic