A preliminary evaluation study of new generation multiplex STR kits comprising of the CODIS core loci and the European Standard Set loci

Jolena Ying Ying Tan; Yuen Peng Tan; Shilen Ng; Annabel Suan Tay; Yong Han Phua; Wei Jie Tan; Terenze Yao Rui Ong; Li Min Chua; Christopher Kiu Choong Syn

doi:10.1016/j.jflm.2017.07.017

A preliminary evaluation study of new generation multiplex STR kits comprising of the CODIS core loci and the European Standard Set loci

J Forensic Leg Med. 2017 Nov:52:16-23. doi: 10.1016/j.jflm.2017.07.017. Epub 2017 Jul 25.

Authors

Jolena Ying Ying Tan¹, Yuen Peng Tan², Shilen Ng², Annabel Suan Tay², Yong Han Phua², Wei Jie Tan², Terenze Yao Rui Ong², Li Min Chua², Christopher Kiu Choong Syn²

Affiliations

¹ DNA Profiling Laboratory, Applied Sciences Group, Health Sciences Authority, 11 Outram Road, 169078, Singapore. Electronic address: jolena_tan@hsa.gov.sg.
² DNA Profiling Laboratory, Applied Sciences Group, Health Sciences Authority, 11 Outram Road, 169078, Singapore.

PMID: 28797659
DOI: 10.1016/j.jflm.2017.07.017

Abstract

The GlobalFiler™ (Life Technologies), Investigator^® 24plex QS (Qiagen), and PowerPlex^® Fusion 6C (Promega) kits are the latest generation 6-dye fluorescent chemistry STR-PCR amplification kits. These kits allow for the simultaneous amplification of the CODIS core loci and the European Standard Set loci, as well as a few Y-STR loci in addition to the standard sex-determining marker Amelogenin. The present study was designed to be a preliminary evaluation of the three STR-PCR kits in terms of sensitivity, profile recovery from degraded DNA samples, tolerance to PCR inhibitors, and detection of minor components in DNA mixtures. The results showed that the three STR-PCR kits had relatively similar performance with each kit faring better for the different aspects studied. The PowerPlex^® Fusion 6C and the Investigator^® 24plex QS kits were shown to tolerate inhibitors better, while the GlobalFiler™ kit appeared to have a higher mean percentage recovery of alleles from low template DNA samples and for minor components in DNA mixtures.

Keywords: DNA profiling; Evaluation study; GlobalFiler™; Investigator(®) 24plex QS; PowerPlex(®) Fusion 6C.

Publication types

Evaluation Study

MeSH terms

Alleles
Amelogenin
Chromosomes, Human, Y
DNA Degradation, Necrotic
DNA Fingerprinting*
Female
Humans
Male
Microsatellite Repeats*
Polymerase Chain Reaction / instrumentation*

Substances

Amelogenin