Detection of human neuronal α7 nicotinic acetylcholine receptors by conjugates of snake α-neurotoxin with quantum dots

Dokl Biochem Biophys. 2017 Jul;475(1):253-255. doi: 10.1134/S1607672917040032. Epub 2017 Sep 2.

Abstract

Fluorescent derivatives are widely used to study the structure and functions of proteins. Quantum dots (QDs), fluorescent semiconductor nanocrystals, have a high quantum yield and are much more resistant to bleaching compared to organic dyes. Conjugates of α-neurotoxins with QDs were used for visualization of human α7 acetylcholine receptors heterologously expressed in GH4C1 pituitary adenoma cells. Specific staining of cells by the conjugated toxins was observed.

MeSH terms

  • Cell Line, Tumor
  • Humans
  • Molecular Imaging
  • Neurotoxins / chemistry*
  • Neurotoxins / metabolism*
  • Quantum Dots / chemistry*
  • Snake Venoms / chemistry*
  • alpha7 Nicotinic Acetylcholine Receptor / metabolism*

Substances

  • Neurotoxins
  • Snake Venoms
  • alpha7 Nicotinic Acetylcholine Receptor