There is a rapidly growing interest in generation of 3D organotypic microtissues with human physiologically relevant structure, function, and cell population in a wide range of applications including drug screening, in vitro physiological/pathological models, and regenerative medicine. Here, we provide a detailed procedure to generate structurally defined 3D organotypic microtissues from cells or cell spheroids using acoustic waves as a biocompatible and scaffold-free tissue engineering tool.
Keywords: 3D organotypic microtissues; Acoustic assembly; Acoustic waves; Cardiac scaffold; Cardiomyocyte; Cell assembly; Cell spheroids; In vitro tissue model; Regenerative medicine; Spheroid assembly; Tissue engineering.