Structural and biochemical changes in dermis of sea cucumber (Stichopus japonicus) during autolysis in response to cutting the body wall

Abstract

The autolysis of sea cucumber body wall is caused by endogenous proteolysis of its structural elements. However, changes in collagen fibrils, collagen fibres and microfibrils, the major structural elements in sea cucumber body wall during autolysis are less clear. Autolysis of sea cucumber (S. japonicus) was induced by cutting the body wall, and the structural and biochemical changes in its dermis were investigated using electron microscopy, differential scanning calorimetry, infrared spectroscopy, electrophoresis, and chemical analysis. During autolysis, both collagen fibres and microfibrils gradually degraded. In contrast, damage to microfibrils was more pronounced. Upon massive autolysis, collagen fibres disaggregated into collagen fibril bundles and individual fibrils due to the fracture of interfibrillar bridges. Meanwhile, excessive unfolding of collagen fibrils occurred. However, there was only slight damage to collagen monomers. Therefore, structural damage in collagen fibres, collagen fibrils and microfibrils rather than monomeric collagen accounts for autolysis of S. japonicus dermis.

Keywords: Acetic acid (PubChem CID: 176); Autolysis; Chondroitin sulphate (PubChem CID: 24766); Collagen fibres; Collagen fibrils; Dimethylmethylene blue (PubChem CID: 123898); Formic acid (PubChem CID: 284); Glutaraldehyde (PubChem CID: 3485); Microfibrils; Sea cucumber (Stichopus japonicus); Sodium cacodylate (PubChem CID: 2724247); Toluidine blue (PubChem CID: 7083); Uranyl acetate (PubChem CID: 10915).