The value of molecular techniques to diagnose Ureaplasma urealyticum and Nocardia farcinica pleuropneumonia in a patient with diffuse large B-cell lymphoma

Etienne Canouï; Kim Blanc; Julien Loubinoux; Sandrine Valade; Cécile Hamard; Aurélie Lefebvre; Sandy Amorim; Cécile Bébéar; Veronica Rodriguez-Nava; David Lebeaux; Odile Launay; Marco Alifano; Antoine Rabbat; Solen Kernéis

doi:10.1016/j.ijid.2017.09.015

The value of molecular techniques to diagnose Ureaplasma urealyticum and Nocardia farcinica pleuropneumonia in a patient with diffuse large B-cell lymphoma

Int J Infect Dis. 2017 Nov:64:93-95. doi: 10.1016/j.ijid.2017.09.015. Epub 2017 Sep 22.

Authors

Affiliations

¹ Université Paris Descartes, Sorbonne Paris Cité, Paris, France; Assistance Publique-Hôpitaux de Paris, Hôpitaux Universitaires Paris Centre-Cochin, Fédération d'Infectiologie, Paris, France. Electronic address: etienne.canoui@aphp.fr.
² Université Paris Descartes, Sorbonne Paris Cité, Paris, France; Assistance Publique-Hôpitaux de Paris, Hôpitaux Universitaires Paris Centre-Cochin, Unité de Soins Intensifs Respiratoires, Paris, France.
³ Université Paris Descartes, Sorbonne Paris Cité, Paris, France; Assistance Publique-Hôpitaux de Paris, Hôpitaux Universitaires Paris Centre-Cochin, Bactériologie, Paris, France.
⁴ Assistance Publique-Hôpitaux de Paris, Hôpital Saint Louis, Réanimation Médicale, Paris, France.
⁵ Assistance Publique-Hôpitaux de Paris, Hôpitaux Universitaires Paris Centre-Cochin, Fédération d'Infectiologie, Paris, France.
⁶ Assistance Publique-Hôpitaux de Paris, Hôpital Saint Louis, Service d'Hémato-oncologie, Paris, France.
⁷ CHU et Université de Bordeaux, Bactériologie, Bordeaux, France.
⁸ Research Group on Bacterial Opportunistic Pathogens and Environment, UMR 5557, Ecologie Microbienne, French Observatory of Nocardiosis, Université de Lyon 1, CNRS, VetAgro Sup, Northern Hospital Group, Hospices Civils de Lyon, Lyon, France.
⁹ Université Paris Descartes, Sorbonne Paris Cité, Paris, France; Assistance Publique-Hôpitaux de Paris, Hôpital Européen Georges Pompidou, Service de Microbiologie, Unité Mobile de Microbiologie Clinique, Paris, France.
¹⁰ Université Paris Descartes, Sorbonne Paris Cité, Paris, France; Assistance Publique-Hôpitaux de Paris, Hôpitaux Universitaires Paris Centre-Cochin, Fédération d'Infectiologie, Paris, France.
¹¹ Université Paris Descartes, Sorbonne Paris Cité, Paris, France; Assistance Publique-Hôpitaux de Paris, Hôpitaux Universitaires Paris Centre-Cochin, Chirurgie Thoracique, Paris, France.
¹² Université Paris Descartes, Sorbonne Paris Cité, Paris, France; Assistance Publique-Hôpitaux de Paris, Hôpitaux Universitaires Paris Centre-Cochin, Fédération d'Infectiologie, Paris, France; INSERM 1181 Biostatistics, Biomathematics, Pharmacoepidemiology, and Infectious Diseases (B2PHI), Paris, France.

PMID: 28951103
DOI: 10.1016/j.ijid.2017.09.015

Abstract

An unusual case of pleural empyema related to Nocardia farcinica and Ureaplasma urealyticum, occurring after autologous haematopoietic stem cell transplantation in a 30-year-old patient with lymphoma, is reported. This case illustrates the role of repeated and comprehensive microbiological investigations and the contribution of molecular techniques in reaching the aetiological diagnosis.

Keywords: Immunocompromised patient; Molecular microbiological diagnosis; Nocardia farcinica; Pleuropneumonia; Ureaplasma urealyticum.

Publication types

Case Reports

MeSH terms

Adult
DNA, Bacterial / analysis
Humans
Lymphoma, B-Cell / complications*
Male
Molecular Typing
Nocardia
Nocardia Infections / complications
Nocardia Infections / diagnosis*
Nocardia Infections / diagnostic imaging
Nocardia Infections / microbiology
Pleuropneumonia / complications
Pleuropneumonia / diagnosis*
Pleuropneumonia / microbiology
Polymerase Chain Reaction*
Positron Emission Tomography Computed Tomography*
RNA, Ribosomal, 16S / analysis
Ureaplasma Infections / complications
Ureaplasma Infections / diagnosis*
Ureaplasma urealyticum* / genetics

Substances

DNA, Bacterial
RNA, Ribosomal, 16S