MicroRNA-205 Mediates Proteinase-Activated Receptor 2 (PAR2) -Promoted Cancer Cell Migration

Cancer Invest. 2017 Oct 21;35(9):601-609. doi: 10.1080/07357907.2017.1378671. Epub 2017 Oct 9.

Abstract

Activation of proteinase-activated receptor 2 (PAR2) promotes cell migration in cancers, but the exact mechanism underlying this process remains largely unknown. Here we report that activation of PAR2 reduced miR-205 expression, whereas inhibition of miR-205 promoted cell migration in cancer cells. Overexpression of miR-205 blocked PAR2-mediated stimulation of cell migration. BMPR1B was identified as a downstream target gene of miR-205. In colorectal carcinoma specimens from patients, the level of PAR2 was negatively correlated with that of miR-205, but it was positively associated with BMPR1B expression. Taken together, our findings indicate that PAR2 signaling promotes cancer cell migration through miR-205/BMPR1B pathway in human colorectal carcinoma.

Keywords: BMPR1B; Colorectal cancer; Migration; PAR2; miR-205.

MeSH terms

  • A549 Cells
  • Bone Morphogenetic Protein Receptors, Type I / genetics
  • Bone Morphogenetic Protein Receptors, Type I / metabolism
  • Cell Movement*
  • Colorectal Neoplasms / genetics
  • Colorectal Neoplasms / metabolism*
  • Colorectal Neoplasms / pathology
  • Gene Expression Regulation, Neoplastic
  • HEK293 Cells
  • HT29 Cells
  • Humans
  • MicroRNAs / genetics
  • MicroRNAs / metabolism*
  • Receptor, PAR-2
  • Receptors, G-Protein-Coupled / genetics
  • Receptors, G-Protein-Coupled / metabolism*
  • Signal Transduction

Substances

  • F2RL1 protein, human
  • MIRN205 microRNA, human
  • MicroRNAs
  • Receptor, PAR-2
  • Receptors, G-Protein-Coupled
  • BMPR1B protein, human
  • Bone Morphogenetic Protein Receptors, Type I