Collagen gel droplet-embedded culture drug sensitivity test for adjuvant chemotherapy after complete resection of non-small-cell lung cancer

Surg Today. 2018 Apr;48(4):380-387. doi: 10.1007/s00595-017-1594-7. Epub 2017 Oct 9.

Abstract

Purpose: We conducted a prospective clinical study to individualize adjuvant chemotherapy after complete resection of non-small-cell lung cancer (NSCLC), based on the drug sensitivity test.

Methods: Patients with resectable c-stage IB-IIIA NSCLC were registered between 2005 and 2010. We performed the collagen gel droplet-embedded culture drug sensitivity test (CD-DST) on a fresh surgical specimen to assess in vitro chemosensitivity and evaluated the prognostic outcome after adjuvant chemotherapy with carboplatin/paclitaxel based on the CD-DST.

Results: Among 92 registered patients, 87 were eligible for inclusion in the analysis. The success rate of CD-DST was 86% and chemosensitivity to carboplatin and/or paclitaxel was evident in 57 (76%) of the 75 patients. Adjuvant chemotherapy was completed in 22 (73%) of 30 patients. The 5-year overall survival rates were 71, 73, and 75% for all, CD-DST success, and chemosensitive patients, respectively. The 5-year disease-free survival and overall survival rates of the chemosensitive patients who completed adjuvant chemotherapy using carboplatin/paclitaxel were 68 and 82%, respectively. The 5-year disease-free survival and overall survival rates of the patients with stage II-IIIA chemosensitive NSCLC were 58 and 75%, respectively. Comparative analyses of the chemosensitive and non-chemosensitive/CD-DST failure groups showed no significant survival difference.

Conclusions: CD-DST can be used to evaluate chemosensitivity after lung cancer surgery; however, its clinical efficacy for assessing individualized treatment remains uncertain.

Keywords: Adjuvant chemotherapy; Drug sensitivity test; Lung cancer; Surgery.

Publication types

  • Clinical Trial, Phase II

MeSH terms

  • Adult
  • Aged
  • Carboplatin / pharmacology*
  • Carcinoma, Non-Small-Cell Lung / mortality
  • Carcinoma, Non-Small-Cell Lung / pathology*
  • Carcinoma, Non-Small-Cell Lung / surgery*
  • Chemotherapy, Adjuvant*
  • Collagen*
  • Culture Media*
  • Culture Techniques / methods*
  • Drug Screening Assays, Antitumor / methods*
  • Drug Therapy, Combination
  • Female
  • Gels
  • Humans
  • Lung Neoplasms / mortality
  • Lung Neoplasms / pathology*
  • Lung Neoplasms / surgery*
  • Male
  • Middle Aged
  • Neoplasm Staging
  • Paclitaxel / pharmacology*
  • Pneumonectomy
  • Precision Medicine
  • Prospective Studies
  • Survival Rate
  • Treatment Outcome

Substances

  • Culture Media
  • Gels
  • Collagen
  • Carboplatin
  • Paclitaxel