Attempt to detect recombination between B-F and B-L genes within the chicken B complex by serological typing, in vitro MLR, and RFLP analyses

Immunogenetics. 1988;28(6):433-8. doi: 10.1007/BF00355375.

Abstract

In search for recombinants within the chicken major histocompatibility B complex, 1155 animals from crosses between the congenic lines CB (B12) and CC (B4) were tested with alloantibodies and monoclonal antibodies for the B-F (class I), B-L (class II), and B-G (class IV) antigens and by mixed lymphocyte reaction. The absence of detectable recombination was confirmed by restriction fragment length polymorphism analysis with B-L beta and B-F probes. Together with previous reports, this indicates that the distance between the B-F and B-L loci is below 0.01 centimorgan.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blood Grouping and Crossmatching*
  • Chickens
  • DNA Probes
  • Female
  • Genes, MHC Class II*
  • Genetic Linkage
  • Genotype
  • Histocompatibility Antigens Class II / genetics*
  • Histocompatibility Antigens Class II / isolation & purification
  • Lymphocyte Culture Test, Mixed*
  • Male
  • Polymorphism, Genetic*
  • Polymorphism, Restriction Fragment Length*
  • Recombination, Genetic*

Substances

  • DNA Probes
  • Histocompatibility Antigens Class II
  • Ia-like antigens, avian