Serum levels of Growth Arrest-Specific 6 protein and soluble AXL in patients with ST-segment elevation myocardial infarction

Eur Heart J Acute Cardiovasc Care. 2019 Dec;8(8):708-716. doi: 10.1177/2048872617740833. Epub 2017 Nov 9.

Abstract

Background: Serum soluble AXL (sAXL) and its ligand, Growth Arrest-Specific 6 protein (GAS6), intervene in tissue repair processes. AXL is increased in end-stage heart failure, but the role of GAS6 and sAXL in ST-segment elevation myocardial infarction (STEMI) is unknown.

Objectives: To study the association of sAXL and GAS6 acutely and six months following STEMI with heart failure and left ventricular remodelling.

Methods: GAS6 and sAXL were measured by enzyme-linked immunosorbent assay at one day, seven days and six months in 227 STEMI patients and 20 controls. Contrast-enhanced magnetic resonance was performed during admission and at six months to measure infarct size and left ventricular function.

Results: GAS6, but not sAXL, levels during admission were significantly lower in STEMI than in controls. AXL increased progressively over time (p<0.01), while GAS6 increased only from day 7. GAS6 or sAXL did not correlate with brain natriuretic peptide or infarct size. However, patients with heart failure (Killip >1) had higher values of sAXL at day 1 (48.9±11.9 vs. 44.0±10.7 ng/ml; p<0.05) and at six months (63.3±15.4 vs. 55.9±13.7 ng/ml; p<0.05). GAS6 levels were not different among subjects with heart failure or left ventricular remodelling. By multivariate analysis including infarct size, Killip class and sAXL at seven days, only the last two were independent predictors of left ventricular remodelling (odds ratio 2.24 (95% confidence interval: 1.08-4.63) and odds ratio 1.04 (95% confidence interval: 1.00-1.08) respectively).

Conclusion: sAXL levels increased following STEMI. Patients with heart failure and left ventricular remodelling have higher sAXL levels acutely and at six month follow-up. These findings suggest a potential role of the GAS6-AXL system in the pathophysiology of left ventricular remodelling following STEMI.

Keywords: AXL receptor tyrosine kinase; GAS6; cardiac remodelling; heart failure; myocardial infarction.

Publication types

  • Comparative Study

MeSH terms

  • Aged
  • Axl Receptor Tyrosine Kinase
  • Female
  • Follow-Up Studies
  • Heart Failure / epidemiology
  • Heart Failure / metabolism
  • Heart Failure / physiopathology
  • Humans
  • Intercellular Signaling Peptides and Proteins / blood*
  • Magnetic Resonance Imaging / methods
  • Male
  • Middle Aged
  • Myocardial Infarction / diagnostic imaging*
  • Myocardial Infarction / metabolism
  • Myocardial Infarction / pathology
  • Myocardial Infarction / physiopathology
  • Natriuretic Peptide, Brain / metabolism
  • Proto-Oncogene Proteins / blood*
  • Receptor Protein-Tyrosine Kinases / blood*
  • ST Elevation Myocardial Infarction / blood*
  • ST Elevation Myocardial Infarction / complications
  • ST Elevation Myocardial Infarction / diagnostic imaging*
  • ST Elevation Myocardial Infarction / pathology
  • Ventricular Remodeling / physiology

Substances

  • Intercellular Signaling Peptides and Proteins
  • Proto-Oncogene Proteins
  • growth arrest-specific protein 6
  • Natriuretic Peptide, Brain
  • Receptor Protein-Tyrosine Kinases
  • Axl Receptor Tyrosine Kinase
  • AXL protein, human