Serum Lipid and Protein Changes in Healthy Dyslipidemic Subjects Given a Selective Inhibitor of p70 S6 Kinase-1

J Clin Pharmacol. 2018 Apr;58(4):412-424. doi: 10.1002/jcph.1032. Epub 2017 Nov 26.

Abstract

The safety, pharmacokinetic, and pharmacodynamic effects of LY2584702, a selective inhibitor for p70 S6 serine/threonine protein kinase-1, were evaluated in healthy dyslipidemic volunteers. LY2584702 was tolerated well as a monotherapy and dose-dependently reduced low-density lipoprotein cholesterol and triglycerides by up to 60% and 50%, respectively, without significantly changing high-density lipoprotein cholesterol levels in plasma. LY2584702 also dose-dependently decreased factor V activity. Alanine aminotransferase elevations were noted in 2 subjects when LY2584702 was given with atorvastatin. We suspect that the formation of 4-aminopyrazolo[3,4-d]pyrimidine (4-APP) during metabolism may have contributed to some of the adverse effects of LY2584702, and the contribution of 4-APP to the pharmacology merits further investigation. Although clinical investigation of LY2584702 has been terminated because of hepatotoxicity risk, we suggest that a selective inhibitor of p70 S6 serine/threonine protein kinase-1 with a larger margin of safety and without the possibility of being metabolized to 4-APP may be useful in the treatment of dyslipidemia.

Keywords: 4-aminopyrazolo[3,4-d]pyrimidine (4-APP); LDL-cholesterol; LY2584702 tosylate; dyslipidemia; p70 S6 kinase-1 (p70S6K1).

Publication types

  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenine / analogs & derivatives
  • Adenine / metabolism
  • Adult
  • Aged
  • Alanine Transaminase / blood
  • Cholesterol, LDL / blood*
  • Cross-Over Studies
  • Dyslipidemias / blood*
  • Female
  • Humans
  • Male
  • Middle Aged
  • Protein Kinase Inhibitors / blood
  • Protein Kinase Inhibitors / pharmacology*
  • Pyrazoles / blood
  • Pyrazoles / pharmacology*
  • Pyrimidines / blood
  • Pyrimidines / pharmacology*
  • Ribosomal Protein S6 Kinases, 70-kDa / antagonists & inhibitors*
  • Single-Blind Method
  • Young Adult

Substances

  • Cholesterol, LDL
  • Protein Kinase Inhibitors
  • Pyrazoles
  • Pyrimidines
  • 4-aminopyrazolo(3,4-d)pyrimidine
  • Alanine Transaminase
  • Ribosomal Protein S6 Kinases, 70-kDa
  • LY2584702
  • Adenine