Abstract
We present a method to capture mesenchymal stromal cells (MSCs) by adhesion to extracellular matrix (ECM) molecules under flow conditions. The technique simulates a physiological system and exploits the natural biological interactions of cells, through integrin receptors, with their ECM. The system offers an insight into how MSCs could be targeted/localized to the site of interest (graft) following intravenous injection.
Keywords:
Adhesion; Fibronectin; Laminin; Mesenchymal stromal cell.
MeSH terms
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Cell Adhesion / physiology
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Cell Differentiation / physiology
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Cells, Immobilized / metabolism
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Extracellular Matrix / physiology*
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Fibronectins / metabolism
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Flow Cytometry / methods*
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Fluorescent Antibody Technique
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Humans
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Image Processing, Computer-Assisted
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Integrins / metabolism
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Laminin / metabolism
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Mesenchymal Stem Cells / classification
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Mesenchymal Stem Cells / cytology*
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Mesenchymal Stem Cells / metabolism*
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Microscopy, Video
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RNA
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Single-Cell Analysis
Substances
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Fibronectins
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Integrins
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Laminin
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RNA