A new continuous cell line derived from an untreated human retinoblastoma has been established. This cell line, FMC-RB1 is strongly positive for common acute lymphoblastic leukemia antigen and shows a number of ring chromosomes and two marker chromosomes considered to be derivations of chromosome #17; the nonrandom chromosomal changes associated with retinoblastoma, particularly the loss of a chromosome #13 or the deletion of 13q14 was not observed. The establishment of the cell line initially required the presence of bone marrow stromal cells. Morphologically, this cell line grew as a suspension of small round cells in grape-like clusters with periodic "shedding" of single cells. FMC-RB1 could be cloned in soft agar, even in the absence of bone marrow stromal cells as "feeders", making it suitable for a variety of biological studies.