This work describes the first 30-parameter immunophenotyping of the human dendritic cell (DC) compartment using fluorescent-based flow cytometry. The optimized panel allows for simultaneous detection of 21 myeloid-centric markers distinguishing all canonical DC subsets, with parallel enumeration of monocytes, T and B cells as well as NK cells. Thus, this panel will be useful for extensive phenotyping of immune cells from a variety of human samples limited in size.
Keywords: antigen-presenting cells; dendritic cells; high-dimensional cytometry; human PBMCs; human tissue; immunophenotyping; myeloid cells.
© 2018 International Society for Advancement of Cytometry.