Cellulose digestion is an essential process of termites, and it is accomplished by three types of cellulases. β-Glucosidase (BG), one of the critical cellulases responsible for cellulose degradation and glucose production, has been considered as a potential target for pest management strategies. Previous experiments identified two new endogenous BG homologs, CfBG-Ia and CfBG-Ib, in the digestive system of Coptotermes formosanus Shiraki (Isoptera: Rhinotermitidae). The objectives of this study were to assess the impact of RNA interference on CfBG-Ia and CfBG-Ib expression and on termite survival. We tested the expression profiles of worker termites which were injected with gene-specific double-stranded RNA (dsRNA, targeting one gene at a time) and a dsRNA cocktail (targeting CfBG-Ia and CfBG-Ib simultaneously). The expression of CfBG-Ib showed a sharp decline in both dsCfBG-Ib and dsRNA cocktail treatments. The expression of CfBG-Ia reduced quickly and significantly in the dsRNA cocktail treatment; while in dsCfBG-Ia treatment, it decreased on the fifth day. Results showed that treatment with the dsRNA cocktail caused greater inhibition of the transcript expression and a shorter response time. However, the expression of nontarget BG homologs was increased as the target BG homologs were being repressed during the testing period in dsRNA cocktail treatment. These results demonstrate that targeting cellulase-coding genes may be a potential strategy to inhibit termite digestion process, or at least dsRNA cocktails serve as a means for identifying the most susceptible target gene families or biological processes.