Harmonization of PCR-based detection of intestinal pathogens: experiences from the Dutch external quality assessment scheme on molecular diagnosis of protozoa in stool samples

Theo A Schuurs; Rob Koelewijn; Eric A T Brienen; Titia Kortbeek; Theo G Mank; Bert Mulder; Foekje F Stelma; Lisette van Lieshout; Jaap J van Hellemond

doi:10.1515/cclm-2017-1057

Harmonization of PCR-based detection of intestinal pathogens: experiences from the Dutch external quality assessment scheme on molecular diagnosis of protozoa in stool samples

Clin Chem Lab Med. 2018 Sep 25;56(10):1722-1727. doi: 10.1515/cclm-2017-1057.

Authors

Theo A Schuurs¹, Rob Koelewijn², Eric A T Brienen³, Titia Kortbeek⁴, Theo G Mank⁵, Bert Mulder⁶, Foekje F Stelma⁷, Lisette van Lieshout³, Jaap J van Hellemond²

Affiliations

¹ Centre for Infectious Diseases Friesland, Izore, Leeuwarden, the Netherlands.
² Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Centre, Rotterdam, the Netherlands.
³ Department of Parasitology, Leiden University Medical Center, Leiden, the Netherlands.
⁴ Centre for Infectious Disease Control Netherlands, National Institute for Public Health and the Environment (RIVM), Bilthoven, the Netherlands.
⁵ Regional Laboratory for Medical Microbiology and Public Health, Haarlem, the Netherlands.
⁶ Department of Medical Microbiology, Canisius-Wilhelmina Hospital, Nijmegen, the Netherlands.
⁷ Department of Medical Microbiology, Radboud University Nijmegen Medical Centre, Nijmegen, the Netherlands.

PMID: 29451859
DOI: 10.1515/cclm-2017-1057

No abstract available

Keywords: Cryptosporidium; Dientamoeba fragilis; Entamoeba histolytica; Giardia lamblia; external quality assessment scheme; intestinal protozoa; real-time PCR.

MeSH terms

Animals
Cryptosporidium / genetics
Cryptosporidium / isolation & purification
DNA, Protozoan / genetics
DNA, Protozoan / metabolism*
DNA, Protozoan / standards
Entamoeba histolytica / genetics
Entamoeba histolytica / isolation & purification
Feces / parasitology*
Gastrointestinal Tract / parasitology
Giardia lamblia / genetics
Giardia lamblia / isolation & purification
Humans
Laboratories, Hospital / standards
Netherlands
Parasites / genetics*
Parasites / isolation & purification
Parasitic Diseases / diagnosis
Polymerase Chain Reaction / methods*
Polymerase Chain Reaction / standards
Quality Control
Sensitivity and Specificity

Substances

DNA, Protozoan