Objective: To identify the genetic variation and possible sources of Thelazia callipaeda isolates collected from patients in Zunyi City, Guizhou Province.
Methods: Seven cases of T. callipaeda infection in Zunyi City, 2016 were verified, and DNA (s) were extracted from the T. callipaeda's body collected from the thelaziasis patients. A mitochondrial COX1 fragment was amplified and sequenced. The sequence alignment and phylogenetical analysis were performed to compare the genetic variation of the gene sequence with the homologous sequences downloaded from Genebank.
Results: COX1 genes of T. callipaeda were differed among the samples from the seven cases, which had low variation.
Conclusions: Zunyi City is a new area with endemic of thelaziasis. The isolates from Zunyi City include either Asian origin or European origin of T. callipaeda. Moreover, at least four haplotypes are identified among the seven isolates.
[摘要]目的 通过线粒体DNA测序及亲缘关系分析初步确定贵州省遵义地区结膜吸吮线虫的来源。 方法 采集 2016年贵州省遵义地区7例人体结膜吸吮线虫病例患者中取出的虫体, 提取DNA, PCR 扩增结膜吸吮线虫线粒体DNA COX1序列, 与已发表的结膜吸吮线虫线粒体DNA COX1序列进行比对和系统进化分析。 结果 自结膜吸吮线虫患者所 得虫体扩增的COX1基因存在差异, 人体感染的虫株之间存在较低的遗传变异。 结论 遵义地区是新的结膜吸吮线虫 病流行区, 其结膜吸吮线虫流行株包含了亚洲及欧洲两种来源, 至少有4种单倍体型。.
Keywords: Haplotype; Human thelaziasis; Mitochondrial DNA; Thelazia callipaeda.