Selected reaction monitoring for colorectal cancer diagnosis using a set of five serum peptides identified by BLOTCHIP®-MS analysis

J Gastroenterol. 2018 Nov;53(11):1179-1185. doi: 10.1007/s00535-018-1448-0. Epub 2018 Mar 1.

Abstract

Background: Colorectal cancer (CRC) is one of the most predominant types of cancer, and it is the fourth most common cause of cancer-related death and it is important to diagnose CRC in early stage to decrease the mortality by CRC. In our previous study, we identified a combination of five peptides as a biomarker candidate to diagnose CRC by BLOTCHIP®-MS analysis using a set of healthy control subjects and CRC patients (stage II-IV). The aim of the present study was to validate the serum biomarker peptides reported in our previous study using a second cohort and to establish their potential usefulness in CRC diagnosis.

Methods: A total of 56 patients with CRC (n = 14 each of stages I-IV), 60 healthy controls, and 60 patients with colonic adenoma were included in this study. The five peptides were extracted and analyzed by selected reaction monitoring using ProtoKey® Colorectal Cancer Risk Test Kit (Protosera, Inc., Amagasaki, Japan).

Results: The results clearly showed that the four CRC groups, stages I-IV, could be sufficiently discriminated from the control group and colonic polyp group. This five-peptide set could identify CRC at each stage compared to the control population in this validation cohort, including those with early-stage disease. The AUC values for each stage of CRC compared to the control population were 0.779, 0.946, 0.852, and 0.973 for stages I, II, III, and IV, respectively.

Conclusions: In this case-control validation study, we confirmed high diagnostic performance for CRC using five peptides that were identified in our previous study as serum biomarker candidates for the detection of CRC.

Keywords: BLOTCHIP®-MS analysis; Biomarker; Colorectal cancer; Peptidome.

MeSH terms

  • Aged
  • Aged, 80 and over
  • Biomarkers, Tumor / blood*
  • Blood Specimen Collection / methods
  • Case-Control Studies
  • Colonic Polyps / diagnosis
  • Colorectal Neoplasms / diagnosis*
  • Colorectal Neoplasms / pathology
  • Diagnosis, Differential
  • Early Detection of Cancer / methods
  • Female
  • Humans
  • Male
  • Middle Aged
  • Neoplasm Staging
  • Peptides / blood
  • Proteomics / methods
  • ROC Curve
  • Reagent Kits, Diagnostic

Substances

  • Biomarkers, Tumor
  • Peptides
  • Reagent Kits, Diagnostic