Extraction and identification of antimicrobial peptides from the Canastra artisanal minas cheese

Tatiana Lopes Fialho; Lanna Clícia Carrijo; Marcos Jorge Magalhães Júnior; Maria Cristina Baracat-Pereira; Roberta Hilsdorf Piccoli; Luiz Ronaldo de Abreu

doi:10.1016/j.foodres.2018.02.009

Extraction and identification of antimicrobial peptides from the Canastra artisanal minas cheese

Food Res Int. 2018 May:107:406-413. doi: 10.1016/j.foodres.2018.02.009. Epub 2018 Feb 17.

Authors

Tatiana Lopes Fialho¹, Lanna Clícia Carrijo², Marcos Jorge Magalhães Júnior², Maria Cristina Baracat-Pereira², Roberta Hilsdorf Piccoli³, Luiz Ronaldo de Abreu³

Affiliations

¹ Food Technology Department, Federal University of Viçosa, 36570-000 Viçosa, MG, Brazil. Electronic address: tatilopesfialho@yahoo.com.br.
² Biochemistry and Molecular Biology Department, Federal University of Viçosa, 36570-000 Viçosa, MG, Brazil.
³ Food Science Department, Federal University of Lavras, 37200-000 Lavras, MG, Brazil.

PMID: 29580501
DOI: 10.1016/j.foodres.2018.02.009

Abstract

Canastra artisanal Minas cheese samples were collected in Minas Gerais - Brazil. The samples were evaluated in order to observe the presence of antimicrobial peptides during 30 days of ripening. Soluble peptides extracted from the cheeses were fractionated by reverse phase liquid chromatography and their fractions evaluated for inhibitory action of E. coli. Fractions containing antimicrobial activity were analyzed by MALDI-TOF/TOF and then peptides were sequenced and identified using MASCOT Daemon coupled with UniProt database. The identified peptides were then validated by SCAFFOLD application. The peptides present in fractions with antimicrobial activity were RPKHPIKHQ, RPKHPIKHQG, RPKHPIKHQGLPQ and RPKHPIKHQGLPQE, HQPHQPLPPT and MHQPHQPLPPT. Peptide sequences PKHPIKHQ, RPKHPIKHQG, RPKHPIKHQGLPQ and RPKHPIKHQGLPQE were originated from α_s1-casein and are their fragments belonging to Isracidine, which in turn is a well known antimicrobial peptide. The HQPHQPLPPT and MHQPHQPLPPT peptides were related to β-casein and were isolated in other studies, but their biological activities are still unknown.

Keywords: Bioactive peptide; Dairy products; MALDI-TOF/TOF; Proteolysis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / analysis*
Caseins / analysis*
Cheese / analysis*
Cheese / microbiology*
Escherichia coli / metabolism*
Peptides / analysis
Peptides / metabolism

Substances

Anti-Bacterial Agents
Caseins
Peptides