Signalling cross-talk between nitric oxide and active oxygen in Trifolium repens L. plants responses to cadmium stress

Abstract

The significant influence of ^•NO on the stress response is well established; however, the precise metabolic pathways of ^•NO and RNS under metal stresses remain unclear. Here, the key components of ROS and RNS metabolism under Cd stress were investigated with multi-level approaches using high-quality forage white clover (Trifolium repens L.) plants. For the studied plants, Cd disturbed the redox homeostasis, affected the absorption of minerals, and exacerbated the degree of lipid peroxidation, thus triggering oxidative stress. However, ^•NO was also involved in regulating mineral absorption, ROS-scavenger levels and mRNA expression in Cd-treated white clover plants. In addition, GSNOR activity was up-regulated by Cd with the simultaneous depletion of ^•NO generation and GSNO but was counteracted by the ^•NO donor sodium nitroprusside. Response to Cd-stressed SNOs was involved in generating ONOO^- and NO₂-Tyr in accordance with the regulation of ^•NO-mediated post-translational modifications in the ASC-GSH cycle, selected amino acids and NADPH-generating dehydrogenases, thereby provoking nitrosative stress. Taken together, our data provide comprehensive metabolite evidence that clearly confirms the relationships between ROS and RNS in Cd-stressed plants, supporting their regulatory roles in response to nitro-oxidative stress and providing an in-depth understanding of the interaction between two families subjected to metal stresses.

Keywords: Amino acids; Cadmium stress; Mineral elements; Nitro-oxidative stress; Reactive nitrogen species.