[Gene expression of D-beta-hydroxybutyrate dehydrogenase. III. Molecular cloning of a DNAc]

C R Seances Soc Biol Fil. 1987;181(5):506-12.
[Article in French]

Abstract

In order to continue the molecular studies of D-beta-hydroxybutyrate dehydrogenase (BDH) undertaken in our laboratory for several years, we have initiated a genetic approach which consists in the BDH cDNA cloning from a rat liver cDNA library. The immunoscreening method allowed to isolate a clone which exhibits a DNA insert shorter than the expected full length BDH cDNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Autoradiography
  • Bacteriophage lambda / genetics
  • Cloning, Molecular* / methods
  • DNA / genetics*
  • Electrophoresis, Polyacrylamide Gel
  • Escherichia coli / genetics
  • Gene Expression Regulation*
  • Hydroxybutyrate Dehydrogenase / genetics*
  • Rats

Substances

  • DNA
  • Hydroxybutyrate Dehydrogenase