SnRNP core protein enrichment in the nuclear matrix

Biochem Biophys Res Commun. 1988 May 16;152(3):1383-7. doi: 10.1016/s0006-291x(88)80438-8.

Abstract

The D protein (16 kDa) is part of a protein core, common to U1, U2, U5, U4/U6 small nuclear RNA containing ribonucleoprotein particles. Monoclonal antibodies reactive with the D protein were used in quantitative dot blotting and Western blotting to demonstrate that this protein was a component of salt resistant nuclear structures and was enriched greater than 3 to 5-fold in RNAase-protected nuclear matrix preparations.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antibodies, Monoclonal
  • Cell Nucleus / analysis
  • HeLa Cells / analysis
  • Humans
  • Molecular Weight
  • Ribonucleases / metabolism
  • Ribonucleoproteins / analysis*
  • Ribonucleoproteins, Small Nuclear

Substances

  • Antibodies, Monoclonal
  • Ribonucleoproteins
  • Ribonucleoproteins, Small Nuclear
  • Ribonucleases