Magnetic Nanosensors for the Detection of Oligonucleotide Sequences

Lee Josephson; J Manuel Perez; Ralph Weissleder

doi:10.1002/1521-3773(20010903)40:17<3204::AID-ANIE3204>3.0.CO;2-H

Magnetic Nanosensors for the Detection of Oligonucleotide Sequences

Angew Chem Int Ed Engl. 2001 Sep 3;40(17):3204-3206. doi: 10.1002/1521-3773(20010903)40:17<3204::AID-ANIE3204>3.0.CO;2-H.

Authors

Lee Josephson¹, J Manuel Perez¹, Ralph Weissleder¹

Affiliation

¹ Harvard Medical School MGH Center For Molecular Imaging Research Building 149, 13th Street, Boston, MA 02129 (USA) Fax: (+1) 617-727-5799.

PMID: 29712059
DOI: 10.1002/1521-3773(20010903)40:17<3204::AID-ANIE3204>3.0.CO;2-H

Abstract

Superparamagnetic nanoparticles conjugated to oligonucleotides exert sensitive and reversible effects on the spin-spin relaxation rates of adjacent water protons when the conjugates hybridize to target oligonucleotides (see temperature cycle in the picture). The fluid-phase magnetic sensors provide the basis for detecting specific nucleic acid sequences in turbid media and potentially in tissues in vivo.

Keywords: DNA recognition; magnetic properties; nanostructures; oligonucleotides.