High-speed volumetric imaging of neuronal activity in freely moving rodents

Oliver Skocek; Tobias Nöbauer; Lukas Weilguny; Francisca Martínez Traub; Chuying Naomi Xia; Maxim I Molodtsov; Abhinav Grama; Masahito Yamagata; Daniel Aharoni; David D Cox; Peyman Golshani; Alipasha Vaziri

doi:10.1038/s41592-018-0008-0

High-speed volumetric imaging of neuronal activity in freely moving rodents

Nat Methods. 2018 Jun;15(6):429-432. doi: 10.1038/s41592-018-0008-0. Epub 2018 May 7.

Authors

Oliver Skocek¹, Tobias Nöbauer¹, Lukas Weilguny², Francisca Martínez Traub¹, Chuying Naomi Xia¹, Maxim I Molodtsov², Abhinav Grama^{3

4}, Masahito Yamagata^{3

4}, Daniel Aharoni⁵, David D Cox^{3

4}, Peyman Golshani^{5

6

7}, Alipasha Vaziri^{8

9

10}

Affiliations

¹ Laboratory of Neurotechnology and Biophysics, The Rockefeller University, New York, NY, USA.
² Research Institute of Molecular Pathology, Vienna, Austria.
³ Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA, USA.
⁴ Center for Brain Science, Harvard University, Cambridge, MA, USA.
⁵ Department of Neurology, Integrative Center for Learning and Memory, Brain Research Institute, University of California, Los Angeles, Los Angeles, CA, USA.
⁶ Department of Psychiatry & Biobehavioral Sciences, Integrative Center for Learning and Memory, Brain Research Institute, University of California, Los Angeles, Los Angeles, CA, USA.
⁷ West Los Angeles Virginia Medical Center, Los Angeles, CA, USA.
⁸ Laboratory of Neurotechnology and Biophysics, The Rockefeller University, New York, NY, USA. vaziri@rockefeller.edu.
⁹ Research Institute of Molecular Pathology, Vienna, Austria. vaziri@rockefeller.edu.
¹⁰ The Kavli Neural Systems Institute, The Rockefeller University, New York, NY, USA. vaziri@rockefeller.edu.

Abstract

Thus far, optical recording of neuronal activity in freely behaving animals has been limited to a thin axial range. We present a head-mounted miniaturized light-field microscope (MiniLFM) capable of capturing neuronal network activity within a volume of 700 × 600 × 360 µm³ at 16 Hz in the hippocampus of freely moving mice. We demonstrate that neurons separated by as little as ~15 µm and at depths up to 360 µm can be discriminated.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Hippocampus / cytology*
Hippocampus / physiology*
Intravital Microscopy / instrumentation
Intravital Microscopy / methods
Mice
Miniaturization / instrumentation*
Neurons / physiology*
Optical Imaging / instrumentation
Optical Imaging / methods

Grants and funding

U54 HD087101/HD/NICHD NIH HHS/United States